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MagDot 610 nm CD4 (RPAT4) – 20 Tests

$420.00

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The MagDot is a dual-purpose reagent, it combines quantum dots and superparamagnetic iron oxide particles within the same nanoparticle. Cells labeled with MagDots targeting specific antigens will be both fluorescent and magnetic. Therefore, after labeling cells of interest, one can look at the feed to determine the percent positive fluorescently via flow cytometry, and then place the labeled cells in a magnetic separator and analyze both the magnetic (for the enrichment) and the nonmagnetic (for the depletion) of targeted cells without the need for further fluorescent labeling.

Mouse anti human CD4 antibody, clone RPA-T4 conjugated to this MagDot recognizes human CD4, a 55 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale.

Product Information

Number of tests

20 tests

Dilution*

15-20 µL/million cells

Antibody Concentration

0.08 mg/mL

Host/Isotype

Mouse/IgG1

Particle Concentration

1011 particles/mL

Iron Concentration

0.5 mg/mL

Antibody per MagDot

700-750 molecules/ MagDot

Class

Monoclonal

Type

Antibody

Clone

RPAT4

Immunogen

Human CD4

Conjugate

610 nm MagDot

Form

Liquid

Purification

Purified

Storage Buffer

Borate, pH 7.4 with 0.5% BSA, 0.1% sodium azide

Storage Conditions

4˚C do not freeze

Reactivity

Human

Tested Application

Flow Cytometry and Magnetic Separation

Catalog Number

MagD-CS-610-H4

 

*Suggested working dilution is given as a guide only. It is recommended the user titrate the product for use in their own experiment using appropriate negative and positive controls.
*Time for separation for the labelled cells will depend on magnetic field strength, gradient used and no of particle bound/ cell.

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