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MagDot 575 nm CD3 (UCHT1) – 20 Tests

$420.00

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The MagDot is a dual-purpose reagent, it combines quantum dots and superparamagnetic iron oxide particles within the same nanoparticle. Cells labeled with MagDots targeting specific antigens will be both fluorescent and magnetic. Therefore, after labeling cells of interest, one can look at the feed to determine the percent positive fluorescently via flow cytometry, and then place the labeled cells in a magnetic separator and analyze both the magnetic (for the enrichment) and the nonmagnetic (for the depletion) of targeted cells without the need for further fluorescent labeling.

The UCHT1 antibody conjugated to this MagDot reacts with the 20 kDa CD3ε subunit of the human T cell receptor (TCR)/CD3 complex, which is expressed on the surface of ~95% of mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale.

Product Information

Number of tests

20 tests

Dilution*

15-20 µL/million cells

Antibody Concentration

0.08 mg/mL

Host/Isotype

Mouse/IgG1

Particle Concentration

1011 particles/mL

Iron Oxide Concentration

0.5 mg/mL

Antibody per MagDot

700-750 molecules/MagDot

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Immunogen

Human CD3

Conjugate

575 nm MagDot

Form

Liquid

Purification

Purified

Storage Buffer

Borate, pH 7.4 with 0.5% BSA, 0.1% sodium azide

Storage Conditions

4˚C do not freeze

Reactivity

Human

Tested Application

Flow Cytometry and Magnetic Separation

Catalog Number

MagD-CS-575-H3

 

*Suggested working dilution is given as a guide only. It is recommended the user titrate the product for use in their own experiment using appropriate negative and positive controls.
*Time for separation for the labelled cells will depend on magnetic field strength, gradient used and no of particle bound/ cell.

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